BioHeme

The development of an efficient pilot-scale leghemoglobine production technology, based on recombinant Pichia pastoris and Kluyveromyces lactis fed-batch fermentations.

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Objective: is to develop effective fed-batch fermentation and scale-up techniques for the production of plant-based meat substitutes, utilizing a recombinant LegH (leghemoglobine) producing strain of Pichia pastoris and Kluyveromyces lactis

The main activities of the project include the creation of two genetically modified yeast strains capable of utilizing cheap and freely available substrates for hemoprotein biosynthesis. As well as to develop the following: efficient fermentation technologies for both previously obtained yeast strains; mathematical models of processes; efficient model-based control systems that would allow automatic control of hemeprotein biosynthesis processes in both batch and fed-batch mode. In addition, the project plans to perform theoretical and practical scaling of hemeprotein production processes to a pilot scale, as well as to evaluate the commercialization potential of the developed fermentation process and related systems.

Grant No. 1.1.1.1/21/A/044

Source of funding: European Union’s Horizon 2020 Research and Innovation Programme

Project period: 01.01.2022 – 31.11.2023

Total budget: 540 540 EUR

Project coordinator: Senior Project Manager, Professor at Latvian State Institute of Wood Chemistry, PhD Juris Vanags, Juris.Vanags@bioreactors.net