Objective: is to develop effective fed-batch fermentation and scale-up techniques for the production of plant-based meat substitutes, utilizing a recombinant LegH (leghemoglobine) producing strain of Pichia pastoris and Kluyveromyces lactis
The main activities of the project include the creation of two genetically modified yeast strains capable of utilizing cheap and freely available substrates for hemoprotein biosynthesis. As well as to develop the following: efficient fermentation technologies for both previously obtained yeast strains; mathematical models of processes; efficient model-based control systems that would allow automatic control of hemeprotein biosynthesis processes in both batch and fed-batch mode. In addition, the project plans to perform theoretical and practical scaling of hemeprotein production processes to a pilot scale, as well as to evaluate the commercialization potential of the developed fermentation process and related systems.
Grant No. 184.108.40.206/21/A/044
Source of funding: European Union’s Horizon 2020 Research and Innovation Programme
Project period: 01.01.2022 – 31.11.2023
Total budget: 540 540 EUR
Project coordinator: Senior Project Manager, Professor at Latvian State Institute of Wood Chemistry, PhD Juris Vanags, Juris.Vanags@bioreactors.net